La propagation des bactéries pathogènes dans l'environnement et la contamination des eaux souterraines

Pili implication from pathogenic bacteria into groundwater contamination

• Auteur / Autrice : Hongbao Wang
• Direction : Edvina Lamy, Yannick Rossez
• Type : Projet de thèse
• Discipline(s) : Génie des procédés
• Date : Inscription en doctorat le 25/09/2024
• Etablissement(s) : Compiègne
• Ecole(s) doctorale(s) : Sciences pour l'ingénieur
• Partenaire(s) de recherche : Laboratoire : Transformations intégrées de la matière renouvelable

Mots clés

Mots clés libres

Contamination

Eau

Transport bactérien

Milieu poreux

Métabolomique

Adhésion

Bacterial transport

Adhesion

Metabolomics

Contamination

Water

Porous media

Résumé

The understanding of the mechanisms governing water flow and transport of pathogenic/indicator microorganisms from the soil and the vadose zone to groundwater will be investigated during this research work.To survive in the environment, bacteria need to adhere first to the surfaces via dedicated appendages like pili or flagella. Recently, the type IV pili has been highlighted to play a crucial role for bacterial survival and spreading in water. So far nothing is known about the role of this appendage for Acinetobacter spreading in the environment and their role into groundwater contamination. For this purpose, lab scale infiltration/percolation experiments at Darcy scale will be performed in soil with distinct pore size distribution in order to investigate and quantify contaminant and microorganisms transport process under various hydrodynamic (saturated and unsaturated) and hydraulic (various flow rates) flow conditions. In the frame of climate change the temperature impact on microbial growth and transport could be investigated by using mutants resisting under various temperatures changes. To provide a better understanding of the mechanisms involved on microorganisms transport and deposition, numerical simulations with HYDRUS code will be performed to characterize water flow (tracer tests) and to estimate microorganisms transport and deposition parameters, necessary to elucidate the mechanisms involved in microorganism's spreding in soil/groundwater environment.