Estuaries and coastal areas are essential fish habitat as nursery and spawning but characterized by the presence of multiple interacting stressors, both natural and anthropogenic, which can represent potential threat toward aquatic organisms, especially for commercial fish species. In this context, the impacts of environmental stressors such as chemical contamination and Harmful algal blooms (HABs) have been studied by in situ and experimentally (microcosm and mesocosm) approaches on two fish species : European flounder (Platichthys flesus) and European sea bass (Dicentrarchus labrax). During this thesis, we used various indicators to determine fish responses to environmental stressors. Among these tools, we used the growth and conditions indices, molecular biomarkers and immunological parameters. In situ approach has been realized in two different systems anthropogenically influenced : one is heavily impacted system (Seine estuary) and the others are less impacted and/or considered as "clean" systems (Canche, Authie and Somme estuaries). As juvenile flounders concentrate in estuaries, we have chosen this species as a biological indicator to evaluate the quality of these estuarine habitats. This in situ study emphasized the negative impact of contaminants on the nursery function of estuaries. The Seine estuary exhibited the highest metals and PAHs contents in sediment compared to other estuaries and metal concentration in juvenile flounder of this estuary were also significantly higher than ones collected in the less polluted estuaries. In the same way, fish growth and condition indices were significantly lower in individuals from this estuary in spite of the sufficient food availability. To control environmental parameters such as hydrological parameters and food availability, a microcosm experiment was carried out on sea bass juveniles exposed to fresh sediment from five sites with different chemical concentrations using multi-biomarker approaches. After 21 days exposure, no metal accumulation in fish gills and any significant differences on the physiological performances and immune system responses of fish juveniles could be observed. On the other hand, responses of molecular biomarkers, particularly, EROD, GST and CAT activities increase with the chemical contamination gradient after 7days of exposure in sediment. This microcosm study confirmed the sensibility and relativity of short term molecular biomarkers responses to the chemical contamination. These two studies highlighted the complexity of the fish responses to environmental stressor due to the many variable environmental factors in situ and due to the selection of fish species (pelagic or benthic) and the exposure duration in controlled laboratory assays. Beside the impact of pollution on fish, Harmful algal blooms (HABs) are widespread along the eastern English Channel and may alter ecological functions of coastal zones and thus affecting nursery ground and fish populations. Nevertheless, the effects of two recurrent harmful algal blooms : a) Phaegocystis globosa and its degraded form transparent exopolymeric particles (TEP) with foam accumulation and b) Pseudo-nitzschia pseudodelicatissima (exponential versus senescent phase) was investigated on the growth and condition of sea bass juveniles. Both mesocosm experiments exhibited any negative impact on juvenile sea bass physiological performance, hence, survival and recruitment success. In conclusion, the results of this thesis contributed to improve the fish responses with multi-biomarker approaches to monitor and assess the health of fish communities and fish habitat quality, as well as the general ecological status of coastal zones and estuaries against the various environmental stressors.